UBE2D3 Rabbit Polyclonal Antibody
cat.: HA500230
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 17 kDa. |
| Isotype: | IgG |
| Immunogen: | Recombinant full length protein of human UBE2D3. |
| Positive control: | Jurkat cell lysate, 293T cell lysate, MCF-7 cell lysate, rat liver tissue lysate, mouse lung tissue lysate, SW620, mouse brain tissue. |
| Subcellular location: | Cell membrane, Endosome membrane. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:500-1:1,000 1:50-1:100 1:100 |
| Uniprot #: | SwissProt: P61077 Human | P61079 Mouse | P61078 Rat |
| Alternative names: | E2(17)KB3 MGC43926 MGC5416 PRO2116 UB2D3_HUMAN UBC 4/5 UBC4/5 UBC4/5 homolog yeast UBC4/5, S. cerevisiae, homolog of UBCH 5C UBCH5C Ube2d3 Ubiquitin carrier protein Ubiquitin carrier protein D3 Ubiquitin conjugating enzyme E2 17 kDa 3 Ubiquitin conjugating enzyme E2 D3 Ubiquitin conjugating enzyme E2D 3 (homologous to yeast UBC4/5) Ubiquitin conjugating enzyme E2D 3 (UBC4/5 homolog yeast) Ubiquitin conjugating enzyme E2D 3 Ubiquitin protein ligase D3 Ubiquitin-conjugating enzyme E2 D3 Ubiquitin-conjugating enzyme E2(17)KB 3 Ubiquitin-conjugating enzyme E2-17 kDa 3 Ubiquitin-protein ligase D3 |
Images
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Fig1:
Western blot analysis of UBE2D3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500230, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Jurkat cell lysate Lane 2: 293T cell lysate Lane 3: MCF-7 cell lysate |
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Fig2:
Western blot analysis of UBE2D3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500230, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Rat liver tissue lysate Lane 2: Mouse lung tissue lysate |
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Fig3: ICC staining of UBE2D3 in SW620 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500230, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig4: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-UBE2D3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500230, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.