MAT1A Rabbit Polyclonal Antibody
cat.: HA500232
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 44 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human MAT1A aa 370-395. |
| Positive control: | Mouse liver tissue lysate, rat liver tissue lysate, human liver tissue lysate, rat liver tissue, mouse liver tissue. |
| Subcellular location: | Cytosol. |
| Recommended Dilutions:
WB IHC-P |
1:2,000-1:5,000 1:400-1:1,000 |
| Uniprot #: | SwissProt: Q00266 Human | Q91X83 Mouse | P13444 Rat |
| Alternative names: | AdoMet AdoMet synthetase 1 AI046368 Ams MAT MAT I/III MATA1 Methionine adenosyltransferase 1 Methionine adenosyltransferase I, alpha Methionine adenosyltransferase I/III MGC108563 S adenosylmethionine synthetase isoform type 1 SADE SAMS SAMS1 |
Images
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Fig1:
Western blot analysis of MAT1A on different lysates with Rabbit anti-MAT1A antibody (HA500232) at 1/5,000 dilution. Lane 1: mouse liver tissue lysate Lane 2: rat liver tissue lysate Lysates/proteins at 30 µg/Lane. Exposure time: 6 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500232, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 44 kDa |
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Fig2: Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-MAT1A antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500232, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-MAT1A antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500232, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.