MAT1A Rabbit Polyclonal Antibody
cat.: HA500232
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 44 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human MAT1A aa 370-395. |
| Positive control: | Mouse liver tissue lysate, rat liver tissue lysate, human liver tissue lysate, rat liver tissue, mouse liver tissue. |
| Subcellular location: | Cytosol. |
| Recommended Dilutions:
WB IHC-P |
1:2,000-1:5,000 1:400-1:1,000 |
| Uniprot #: | SwissProt: Q00266 Human | Q91X83 Mouse | P13444 Rat |
| Alternative names: | AdoMet AdoMet synthetase 1 AI046368 Ams MAT MAT I/III MATA1 Methionine adenosyltransferase 1 Methionine adenosyltransferase I, alpha Methionine adenosyltransferase I/III MGC108563 S adenosylmethionine synthetase isoform type 1 SADE SAMS SAMS1 |
Images
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Fig1:
Western blot analysis of MAT1A on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500232, 1/5,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse liver tissue lysate Lane 2: Rat liver tissue lysate Lane 3: Human liver tissue lysate |
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Fig2: Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-MAT1A antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500232, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-MAT1A antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500232, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.