RGS6 Rabbit Polyclonal Antibody
cat.: HA500233
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 54 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human RGS6 aa 300-472. |
| Positive control: | Rat brain tissue lysate, mouse brain tissue lysate, Hela cell lysates, Hela. |
| Subcellular location: | Nucleus, Cytoplasm, Cytosol, Cell membrane, Membrane. |
| Recommended Dilutions:
WB FC |
1:2,000-1:10,000 1:500-1:1,000 |
| Uniprot #: | SwissProt: P49758 Human | Q9Z2H2 Mouse | P49801 Rat |
| Alternative names: | FLJ43552 G protein signaling 6 regulator GAP GTPase activating protein MGC142132 Regulator of G protein signaling 6 Regulator of G protein signalling 6 Regulator of G-protein signaling 6 RGS 6 RGS6 RGS6_HUMAN S914 |
Images
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Fig1:
Western blot analysis of RGS6 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500233, 1/10,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Rat brain tissue lysate Lane 2: Mouse brain tissue lysate |
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Fig2: Western blot analysis of RGS6 on Hela cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500233, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
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Fig3: Flow cytometric analysis of RGS6 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (HA500233, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.