RPE Rabbit Polyclonal Antibody
cat.: HA500234
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 25 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human RPE aa 50-228. |
| Positive control: | Mouse heart tissue lysate, human heart tissue lysate, human small intestine tissue, SH-SY5Y. |
| Subcellular location: | Cytosol, extracellular exosome. |
| Recommended Dilutions:
WB IHC-P IF-Cell |
1:2,000-5,000 1:500-1:5,000 1:50-1:100 |
| Uniprot #: | SwissProt: Q96AT9 Human | Q8VEE0 Mouse |
| Alternative names: | 2810429B02Rik 5730518J08Rik EC 5.1.3.1 MGC124653 MGC2636 OTTHUMP00000163939 Ribulose 5 phosphate 3 epimerase Ribulose 5 phosphate epimerase Ribulose phosphate 3 epimerase Ribulose-5-phosphate-3-epimerase Ribulose-phosphate 3-epimerase rpe RPE_HUMAN RPE2 1 |
Images
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Fig1:
Western blot analysis of RPE on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500234, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse heart tissue lysate Lane 2: Human heart tissue lysate |
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Fig2: Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-RPE antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500234, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: ICC staining of RPE in SH-SY5Y cells (green). Methanol fixed cells were blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500234, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.