SMG5 Rabbit Polyclonal Antibody
cat.: HA500236
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 114 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human SMG5 aa 800-1,000. |
| Positive control: | Jurkat cell lysate, Hela cell lysate, 293 cell lysate, THP-1 cell lysate, mouse testis tissue lysates, rat testis tissue lysates, Hela. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB FC |
1:1,000-1:5,000 1:500-1:1,000 |
| Uniprot #: | SwissProt: Q9UPR3 Human | Q6ZPY2 Mouse Entrez Gene: 681012 Rat |
| Alternative names: | Protein SMG5 EST1-like protein B LPTS-RP1 LPTS-interacting protein SMG-5 homolog hSMG-5 SMG5 EST1B KIAA1089 EST1 like protein B EST1B LPTS interacting protein LPTS RP1 Nonsense mediated mRNA decay factor |
Images
|
Fig1:
Western blot analysis of SMG5 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500236, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Jurkat cell lysate Lane 2: Hela cell lysate Lane 3: 293 cell lysate Lane 4: THP-1 cell lysate |
|
Fig2: Western blot analysis of SMG5 on mouse testis tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500236, 1/5,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
|
Fig3: Western blot analysis of SMG5 on rat testis tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500236, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
|
Fig4: Flow cytometric analysis of SMG5 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (HA500236, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.