ChT1 Rabbit Polyclonal Antibody
cat.: HA500237
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 42 kDa. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human VSIG1 aa 50-100 (Extracellular domain). |
| Positive control: | Mouse testis tissue lysate, rat testis tissue lysate, human ovarian carcinoma tissue, human stomach tissue. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:200-1:500 |
| Uniprot #: | SwissProt: Q86XK7 Human | Q9D2J4 Mouse | Q4KLY3 Rat |
| Alternative names: | 1700062D20Rik Cell surface A33 antigen ChT1 thymocyte antigen dJ889N15.1 Glycoprotein A34 GPA34 MGC44287 OTTHUMP00000023820 V set and immunoglobulin domain containing V-set and immunoglobulin domain containing 1 V-set and immunoglobulin domain-containing protein 1 VSIG1 VSIG1_HUMAN |
Images
|
Fig1:
Western blot analysis of ChT1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500237, 1/1,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse testis tissue lysate Lane 2: Rat testis tissue lysate |
|
Fig2: Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue using anti-ChT1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500237, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3: Immunohistochemical analysis of paraffin-embedded human stomach tissue using anti-ChT1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500237, 1/500) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.