Met (c-Met) Rabbit Polyclonal Antibody
cat.: HA500242
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 156 kDa. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Met aa 1350-1390. |
| Positive control: | LO2 cell lysate, Hela cell lysate, A549 cell lysate, A431 cell lysate, HT-29 cell lysate, human lung carcinoma tissue, SiHa. |
| Subcellular location: | Membrane; Secreted. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:500 1:200 1:400 |
| Uniprot #: | SwissProt: P08581 Human |
| Alternative names: | AUTS9 c met D249 Hepatocyte growth factor receptor HGF HGF receptor HGF/SF receptor HGFR MET Met proto oncogene Met proto oncogene tyrosine kinase MET proto oncogene, receptor tyrosine kinase Met proto-oncogene (hepatocyte growth factor receptor) Met proto-oncogene Met protooncogene MET_HUMAN Oncogene MET Par4 Proto-oncogene c-Met RCCP2 Scatter factor receptor SF receptor Tyrosine-protein kinase Met |
Images
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Fig1:
Western blot analysis of Met (c-Met) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500242, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: LO2 cell lysate Lane 2: Hela cell lysate Lane 3: A549 cell lysate Lane 4: A431 cell lysate Lane 5: HT-29 cell lysate |
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Fig2: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-Met (c-Met) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500242, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunocytochemistry analysis of SiHa cells labeling Met (c-Met) with Rabbit anti-Met (c-Met) antibody (HA500242) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Met (c-Met) antibody (HA500242) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) were used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.