ClC2 Rabbit Polyclonal Antibody
cat.: HA500243
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 99 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human ClC2 aa 631-830. |
| Positive control: | HepG2 cell lysate, 293 cell lysate, SiHa, Hela. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IF-Cell FC |
1:500 1:200 1:500-1:1,000 |
| Uniprot #: | SwissProt: P51788 Human | P35525 Rat |
| Alternative names: | Chloride Channel 2 Chloride channel protein 2 Chloride channel, voltage sensitive 2 CIC 2 CIC2 ClC-2 CLC2 Clcn2 CLCN2_HUMAN ECA2 ECA3 EG13 EGI11 EGMA EJM6 EJM8 PKA-activated chloride channel |
Images
|
Fig1:
Western blot analysis of ClC2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500243, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HepG2 cell lysate Lane 2: 293 cell lysate Predicted band size: 99 kDa Observed band size: 99 kDa |
|
Fig2:
Immunocytochemistry analysis of SiHa cells labeling ClC2 with Rabbit anti-ClC2 antibody (HA500243) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-ClC2 antibody (HA500243) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
|
Fig3:
Flow cytometric analysis of Hela cells labeling ClC2. Cells were fixed and permeabilized. Then stained with the primary antibody (HA500243, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.