HA500243

ClC2 Rabbit Polyclonal Antibody

cat.: HA500243

Product Type:	Rabbit polyclonal IgG, primary antibodies
Species reactivity:	Human, Rat
Applications:	WB, IF-Cell, IHC-P, FC
Clonality:	Polyclonal

Form:	Liquid
Storage condition:	Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer:	1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Immunogen affinity purified.
Molecular weight:	Predicted band size: 99 kDa
Isotype:	IgG
Immunogen:	Recombinant protein within human ClC2 aa 631-830.
Positive control:	HepG2 cell lysate, 293 cell lysate, human kidney tissue, human stomach tissue, rat colon tissue, SiHa, Hela.
Subcellular location:	Cell membrane.
Recommended Dilutions: WB IF-Cell IHC-P FC	1:500 1:200 1:200 1:500-1:1,000
Uniprot #:	SwissProt: P51788 Human \| P35525 Rat
Alternative names:	Chloride Channel 2 Chloride channel protein 2 Chloride channel, voltage sensitive 2 CIC 2 CIC2 ClC-2 CLC2 Clcn2 CLCN2_HUMAN ECA2 ECA3 EG13 EGI11 EGMA EJM6 EJM8 PKA-activated chloride channel

Images

	Fig1: Western blot analysis of ClC2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500243, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HepG2 cell lysate Lane 2: 293 cell lysate Predicted band size: 99 kDa Observed band size: 99 kDa
	Fig2: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ClC2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA500243, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig3: Immunohistochemical analysis of paraffin-embedded human stomach tissue using anti-ClC2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA500243, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig4: Immunohistochemical analysis of paraffin-embedded rat colon tissue using anti-ClC2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA500243, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Fig5: Immunocytochemistry analysis of SiHa cells labeling ClC2 with Rabbit anti-ClC2 antibody (HA500243) at 1/200 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-ClC2 antibody (HA500243) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Fig6: Flow cytometric analysis of Hela cells labeling ClC2.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA500243, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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