Product Type: | Rabbit polyclonal IgG, primary antibodies |
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Species reactivity: | Human |
Applications: | WB |
Clonality: | Polyclonal |
Form: | Liquid |
Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Immunogen affinity purified. |
Molecular weight: | Predicted band size: 24 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within human Gankyrin aa 1-226. |
Positive control: | A549 cell lysate, Hela cell lysate, 293T cell lysate. |
Subcellular location: | Cytoplasm, Nucleus. |
Recommended Dilutions:
WB |
1:500 |
Uniprot #: | SwissProt: O75832 Human |
Alternative names: | 26S proteasome non-ATPase regulatory subunit 10 26S proteasome regulatory subunit p28 Ankyrin repeat protein dJ889N15.2 Gankyrin Hepatocellular carcinoma-associated protein p28 II p28 p28(GANK) Proteasome (prosome, macropain) 26S subunit, non ATPase, 10 Proteasome 26S S10 PSD10_HUMAN Psmd10 |
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Fig1:
Western blot analysis of Gankyrin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500244, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: A549 cell lysate Lane 2: Hela cell lysate Lane 3: 293T cell lysate Predicted band size: 24 kDa Observed band size: 24 kDa |