IFNGR2 Rabbit Polyclonal Antibody
cat.: HA500246
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, IF-Tissue |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 38 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human IFN gamma Receptor beta aa 288-337. |
| Positive control: | HeLa cell lysate, C2C12 cell lysate, F9 cell lysate, mouse lung tissue lysate, mouse spleen tissue lysate, rat lung tissue lysate, rat spleen tissue lysate, SH-SY5Y, human stomach tissue, mouse bladder tissue, rat lung tissue. |
| Subcellular location: | Golgi apparatus membrane, Cell membrane, Cytoplasm, Endoplasmic reticulum membrane, Cytoplasmic vesicle membrane. |
| Recommended Dilutions:
WB IF-Cell IHC-P IF-Tissue |
1:1,000 1:200 1:600 1:200 |
| Uniprot #: | SwissProt: P38484 Human Entrez Gene: 15980 Mouse | 360697 Rat |
| Alternative names: | AF 1 AF-1 AF1 IFGR 2 IFGR2 IFN gamma Receptor beta IFN-gamma receptor 2 IFN-gamma-R2 IFNGR 2 IFNGR2 IFNGT 1 IFNGT1 IMD28 INGR2_HUMAN Interferon gamma receptor 2 (interferon gamma transducer 1) Interferon gamma receptor 2 Interferon gamma receptor accessory factor 1 Interferon gamma receptor beta chain Interferon gamma receptor chain 2 Interferon gamma transducer 1 |
Images
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Fig1:
Western blot analysis of IFNGR2 on different lysates with Rabbit anti-IFNGR2 antibody (HA500246) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: C2C12 cell lysate (20 µg/Lane) Lane 3: F9 cell lysate (20 µg/Lane) Lane 4: Mouse lung tissue lysate (40 µg/Lane) Lane 5: Mouse spleen tissue lysate (40 µg/Lane) Lane 6: Rat lung tissue lysate (40 µg/Lane) Lane 7: Rat spleen tissue lysate (40 µg/Lane) Predicted band size: 38 kDa Observed band size: 45 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500246) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining of IFNGR2 in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500246, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3: Immunohistochemical analysis of paraffin-embedded human stomach tissue using anti-IFNGR2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500246, 1/600) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded mouse bladder tissue using anti-IFNGR2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500246, 1/600) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5: Immunohistochemical analysis of paraffin-embedded rat lung tissue using anti-IFNGR2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500246, 1/600) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Application: IF-Tissue Species: Mouse Site: bladder Sample: Paraffin-embedded section Antibody concentration: 1/200 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.