IFN gamma Receptor beta Rabbit Polyclonal Antibody
cat.: HA500246
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 38 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human IFN gamma Receptor beta aa 288-337.
Positive control: HeLa cell lysate, C2C12 cell lysate, F9 cell lysate, mouse lung tissue lysate, mouse spleen tissue lysate, rat lung tissue lysate, rat spleen tissue lysate, SH-SY5Y, human stomach tissue, mouse bladder tissue, rat lung tissue.
Subcellular location: Golgi apparatus membrane, Cell membrane, Cytoplasm, Endoplasmic reticulum membrane, Cytoplasmic vesicle membrane.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
1:1,000
1:200
1:600
Uniprot #: SwissProt: P38484 Human
Entrez Gene: 15980 Mouse | 360697 Rat
Alternative names: AF 1 AF-1 AF1 IFGR 2 IFGR2 IFN gamma Receptor beta IFN-gamma receptor 2 IFN-gamma-R2 IFNGR 2 IFNGR2 IFNGT 1 IFNGT1 IMD28 INGR2_HUMAN Interferon gamma receptor 2 (interferon gamma transducer 1) Interferon gamma receptor 2 Interferon gamma receptor accessory factor 1 Interferon gamma receptor beta chain Interferon gamma receptor chain 2 Interferon gamma transducer 1
Images
HA500246_1.jpg Fig1: Western blot analysis of IFN gamma Receptor beta on different lysates with Rabbit anti-IFN gamma Receptor beta antibody (HA500246) at 1/1,000 dilution.

Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: C2C12 cell lysate (20 µg/Lane)
Lane 3: F9 cell lysate (20 µg/Lane)
Lane 4: Mouse lung tissue lysate (40 µg/Lane)
Lane 5: Mouse spleen tissue lysate (40 µg/Lane)
Lane 6: Rat lung tissue lysate (40 µg/Lane)
Lane 7: Rat spleen tissue lysate (40 µg/Lane)

Predicted band size: 38 kDa
Observed band size: 45 kDa

Exposure time: 1 minute; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500246) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA500246_2.jpg Fig2: ICC staining of IFN gamma Receptor beta in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500246, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
HA500246_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human stomach tissue using anti-IFN gamma Receptor beta antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500246, 1/600) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500246_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse bladder tissue using anti-IFN gamma Receptor beta antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500246, 1/600) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500246_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat lung tissue using anti-IFN gamma Receptor beta antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500246, 1/600) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.