Aquaporin 3 Rabbit Polyclonal Antibody
cat.: HA500247
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 32 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human Aquaporin 3 aa 243-292.
Positive control: Mouse kidney tissue lysates, human esophagus tissue, human kidney tissue, human prostate carcinoma tissue, rat bladder tissue, rat stomach tissue, human lung squamous cell carcinoma tissue, human cervical carcinoma tissue, Hela.
Subcellular location: Cell membrane, Basolateral cell membrane.
Recommended Dilutions:
  WB
  IHC-P
  FC
1:500
1:200-1:600
1:500-1:1,000
Uniprot #: SwissProt: Q92482 Human | Q8R2N1 Mouse | P47862 Rat
Alternative names: AQP 3 AQP-3 Aqp3 AQP3_HUMAN Aquaglyceroporin-3 Aquaporin 3 (GIL blood group) Aquaporin 3 (Gill blood group) Aquaporin-3 Aquaporin3 GIL Gill blood group
Images
HA500247_1.jpg Fig1: Western blot analysis of Aquaporin 3 on mouse kidney tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500247, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

Predicted band size: 32 kDa
Observed band size: 35 kDa
HA500247_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human esophagus tissue using anti-Aquaporin 3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500247, 1/600) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500247_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Aquaporin 3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500247, 1/600) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500247_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue using anti-Aquaporin 3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500247, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500247_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat bladder tissue using anti-Aquaporin 3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500247, 1/600) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500247_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded rat stomach tissue using anti-Aquaporin 3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500247, 1/600) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500247_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue with Rabbit anti-Aquaporin 3 antibody (HA500247) at 1/600 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500247) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500247_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissue with Rabbit anti-Aquaporin 3 antibody (HA500247) at 1/600 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500247) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500247_9.jpg Fig9: Flow cytometric analysis of Hela cells labeling Aquaporin 3.

Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA500247, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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