Peptide YY Rabbit Polyclonal Antibody
cat.: HA500250
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 11 kDa. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within N-terminal human Peptide YY. |
| Positive control: | Human small intestine tissue lysate, RAW264.7 cell lysate, rat liver tissue lysate, SW620, SiHa. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IF-Cell FC |
1:1,000 1:200 1:100-1:500 |
| Uniprot #: | SwissProt: P10082 Human | Q9EPS2 Mouse | P10631 Rat |
| Alternative names: | GHYY MGC19143 Peptide tyrosine tyrosine peptide YY Peptide YY like Peptide YY precursor Peptide YY(3-36) Prepro PYY PYY 1 PYY PYY II PYY-I PYY-II PYY_HUMAN PYY1 RATGHYY Yy |
Images
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Fig1:
Western blot analysis of Peptide YY on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500250, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Human small intestine tissue lysate Lane 2: RAW264.7 cell lysate Lane 1: Rat liver tissue lysate Predicted band size: 11 kDa. Observed band size: 20 kDa. |
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Fig2: ICC staining of Peptide YY in SW620 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500250, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3: Flow cytometric analysis of Peptide YY was done on SiHa cells. The cells were fixed, permeabilized and stained with the primary antibody (HA500250, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.