IL-10RA Rabbit Polyclonal Antibody
cat.: HA500251
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 63 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human IL-10RA aa 491-540/578. |
| Positive control: | Daudi cell lysates, Jurkat cell lysate, THP-1 cell lysate, MCF-7 cell lysate, human tonsil tissue, SiHa. |
| Subcellular location: | Cytoplasm, Cell membrane. |
| Recommended Dilutions:
WB IHC-P IF-Cell |
1:500-1:2,000 1:200-1:1,000 1:50-1:200 |
| Uniprot #: | SwissProt: Q13651 Human |
| Alternative names: | CD210 CDw210a CDw210a antigen HIL 10R HIL10R I10R1_HUMAN IL 10R 1 IL 10R A IL 10R IL 10R1 IL-10 receptor subunit alpha IL-10R subunit 1 IL-10R subunit alpha IL-10R1 IL-10RA IL10R IL10R1 IL10RA Interleukin 10 receptor alpha Interleukin 10 receptor alpha chain Interleukin-10 receptor subunit 1 Interleukin-10 receptor subunit alpha |
Images
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Fig1: Western blot analysis of IL-10RA on Daudi cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500251, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of IL-10RA on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500251, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Jurkat cell lysate Lane 2: THP-1 cell lysate Lane 3: MCF-7 cell lysate |
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Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IL-10RA antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500251, 1/600) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: ICC staining of IL-10RA in SiHa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500251, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.