Granzyme B Rabbit Polyclonal Antibody
cat.: HA500252
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human
Applications: IHC-P, mIHC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: 28 kDa
Isotype: IgG
Immunogen: Recombinant protein within human Granzyme B aa 50-247.
Positive control: human spleen tissue, human cervical cancer.
Subcellular location: Secreted.
Recommended Dilutions:
  IHC-P
  mIHC

1:100-1:500
1:200
Uniprot #: SwissProt: P10144 Human
Alternative names: C11 Cathepsin G like 1 Cathepsin G-like 1 CCPI CGL 1 CGL1 CSP B CSPB CTLA 1 CTLA-1 CTLA1 CTSGL1 Cytotoxic serine protease B Cytotoxic T lymphocyte associated serine esterase 1 Cytotoxic T lymphocyte proteinase 2 Cytotoxic T-lymphocyte proteinase 2 Fragmentin 2 Fragmentin-2 GRAB_HUMAN Granzyme 2 Granzyme B (granzyme 2, cytotoxic T lymphocyte associated serine esterase 1) Granzyme B Granzyme-2 GranzymeB GRB Gzmb Hlp Human lymphocyte protein Lymphocyte protease Protease, serine, B SECT T cell serine protease 1 3E T cell serine protease 1-3E T-cell serine protease 1-3E
Images
HA500252_1.jpg Fig1: Fluorescence multiplex immunohistochemical analysis of tertiary lymphoid structures in human cervical cancer (Formalin/PFA-fixed paraffin-embedded sections). Panel A: the merged image of anti-Granzyme B (HA500252, magenta), anti-CD4 (ET1609-52, yellow) on tertiary lymphoid structures. Panel B: anti- Granzyme B stained on cytotoxic NK cells and dendritic cells. Panel C: anti-CD4 stained on helper T cells and Treg cells. HRP Conjugated UltraPolymer Goat Polyclonal Antibody HA1119/HA1120 was used as a secondary antibody. The immunostaining was performed with the Sequential Immuno-staining Kit (IRISKit™MH010101, www.luminiris.cn). The section was incubated in three rounds of staining: in the order of HA500252 (1/200 dilution), ET1609-52 (1/1,000 dilution) for 20 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95C. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
HA500252_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Granzyme B antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500252, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.