Granzyme B Rabbit Polyclonal Antibody
cat.: HA500252
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IHC-P, mIHC, IF-Tissue |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 28 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Granzyme B aa 50-247. |
| Positive control: | Human liver tissue, human spleen tissue. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
IHC-P mIHC IF-Tissue |
1:3,000 1:200-1:2,000 1:500 |
| Uniprot #: | SwissProt: P10144 Human |
| Alternative names: | C11 Cathepsin G like 1 Cathepsin G-like 1 CCPI CGL 1 CGL1 CSP B CSPB CTLA 1 CTLA-1 CTLA1 CTSGL1 Cytotoxic serine protease B Cytotoxic T lymphocyte associated serine esterase 1 Cytotoxic T lymphocyte proteinase 2 Cytotoxic T-lymphocyte proteinase 2 Fragmentin 2 Fragmentin-2 GRAB_HUMAN Granzyme 2 Granzyme B (granzyme 2, cytotoxic T lymphocyte associated serine esterase 1) Granzyme B Granzyme-2 GranzymeB GRB Gzmb Hlp Human lymphocyte protein Lymphocyte protease Protease, serine, B SECT T cell serine protease 1 3E T cell serine protease 1-3E T-cell serine protease 1-3E |
Images
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Fig1: Fluorescence multiplex immunohistochemical analysis of human tonsil (Formalin/PFA-fixed paraffin-embedded sections). Panel A: the merged image of anti-CD14 (ET1610-85, Green), anti-CD21 (HA721163, Red) and anti-Granzyme B (HA500252, Yellow) on tonsil. HRP Conjugated UltraPolymer Goat Polyclonal Antibody HA1119/HA1120 was used as a secondary antibody. The immunostaining was performed with the Sequential Immuno-staining Kit (IRISKit™MH010101, www.luminiris.cn). The section was incubated in three rounds of staining: in the order of ET1610-85 (1/800 dilution), HA721163 (1/1,000 dilution) and HA500252 (1/200 dilution) for 20 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Zeiss Observer 7 Inverted Fluorescence Microscope. |
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Fig2: Fluorescence multiplex immunohistochemical analysis of tertiary lymphoid structures in human cervical cancer (Formalin/PFA-fixed paraffin-embedded sections). Panel A: the merged image of anti-Granzyme B (HA500252, magenta), anti-CD4 (ET1609-52, yellow) on tertiary lymphoid structures. Panel B: anti- Granzyme B stained on cytotoxic NK cells and dendritic cells. Panel C: anti-CD4 stained on helper T cells and Treg cells. HRP Conjugated UltraPolymer Goat Polyclonal Antibody HA1119/HA1120 was used as a secondary antibody. The immunostaining was performed with the Sequential Immuno-staining Kit (IRISKit™MH010101, www.luminiris.cn). The section was incubated in three rounds of staining: in the order of HA500252 (1/200 dilution), ET1609-52 (1/1,000 dilution) for 20 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner. |
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Fig3:
Application: IF-Tissue Species: Human Site: liver Sample: Paraffin-embedded section Antibody concentration: 1/500 |
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Fig4:
Application: IF-Tissue Species: Human Site: spleen Sample: Paraffin-embedded section Antibody concentration: 1/500 |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Granzyme B antibody (HA500252) at 1/3,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500252) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-Granzyme B antibody (HA500252) at 1/3,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500252) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.