PRDM1 Rabbit Polyclonal Antibody
cat.: HA500255
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 92 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human aa 770-825 / 825. |
| Positive control: | Rat cervix tissue, human skin tissue, human esophagus tissue, mouse esophagus tissue, PANC-1. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
IHC-P FC |
1:100-1:500 1:50-1:100 |
| Uniprot #: | SwissProt: O75626 Human | Q60636 Mouse |
| Alternative names: | B Lymphocyte Induced Maturation Protein 1 Beta interferon gene positive regulatory domain I binding factor Beta-interferon gene positive regulatory domain I-binding factor BLIMP-1 BLIMP1 Positive Regulatory Domain I Binding Factor 1 Positive regulatory domain I-binding factor 1 PR Domain Containing 1 PR domain containing 1 with ZNF domain PR domain containing 1 with ZNF domain isoform 2 PR domain containing protein 1 PR domain zinc finger protein 1 PR domain-containing protein 1 PRDI BF1 PRDI binding factor 1 PRDI-BF1 PRDI-binding factor 1 PRDM 1 Prdm1 PRDM1_HUMAN |
Images
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Fig1: Immunohistochemical analysis of paraffin-embedded rat cervix tissue using anti-PRDM1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500255, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig2: Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-PRDM1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500255, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3: Immunohistochemical analysis of paraffin-embedded human esophagus tissue using anti-PRDM1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500255, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4: Immunohistochemical analysis of paraffin-embedded mouse esophagus tissue using anti-PRDM1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500255, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5: Flow cytometric analysis of PRDM1 was done on PANC-1 cells. The cells were fixed, permeabilized and stained with the primary antibody (HA500255, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.