PRDM1 Rabbit Polyclonal Antibody
cat.: HA500255
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: IHC-P, FC, WB
Clonality: Polyclonal
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 92 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human aa 770-825 / 825.
Positive control: Rat cervix tissue, human skin tissue, human esophagus tissue, mouse esophagus tissue, PANC-1, mouse kidney tissue lysate, rat kidney tissue lysate.
Subcellular location: Cytoplasm, Nucleus.
Recommended Dilutions:
  IHC-P
  FC
  WB
1:100-1:500
1:50-1:100
1:1,000
Uniprot #: SwissProt: O75626 Human | Q60636 Mouse
Alternative names: B Lymphocyte Induced Maturation Protein 1 Beta interferon gene positive regulatory domain I binding factor Beta-interferon gene positive regulatory domain I-binding factor BLIMP-1 BLIMP1 Positive Regulatory Domain I Binding Factor 1 Positive regulatory domain I-binding factor 1 PR Domain Containing 1 PR domain containing 1 with ZNF domain PR domain containing 1 with ZNF domain isoform 2 PR domain containing protein 1 PR domain zinc finger protein 1 PR domain-containing protein 1 PRDI BF1 PRDI binding factor 1 PRDI-BF1 PRDI-binding factor 1 PRDM 1 Prdm1 PRDM1_HUMAN
Images
HA500255_1.jpg Fig1: Immunohistochemical analysis of paraffin-embedded rat cervix tissue using anti-PRDM1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500255, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500255_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-PRDM1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500255, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500255_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human esophagus tissue using anti-PRDM1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500255, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500255_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse esophagus tissue using anti-PRDM1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500255, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500255_5.jpg Fig5: Flow cytometric analysis of PRDM1 was done on PANC-1 cells. The cells were fixed, permeabilized and stained with the primary antibody (HA500255, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
HA500255_6.jpg Fig6: Western blot analysis of PRDM1 on different lysates with Rabbit anti-PRDM1 antibody (HA500255) at 1/1,000 dilution.

Lane 1: Mouse kidney tissue lysate
Lane 2: Rat kidney tissue lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 92 kDa
Observed band size: 75 kDa

Exposure time: 3 minutes; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500255) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.