GNG11 Rabbit Polyclonal Antibody
cat.: HA500256
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 8 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human GNG11 aa 1-73. |
| Positive control: | Human skeletal muscle tissue lysates, Hela, SH-SY5Y. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IF-Cell FC |
1:500 1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P61952 Human | P61953 Mouse | P61954 Rat |
| Alternative names: | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-11 GNG11 GNGT11 |
Images
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Fig1:
Western blot analysis of GNG11 on human skeletal muscle tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500256, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Predicted band size: 8 kDa Observed band size: 11 kDa |
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Fig2: ICC staining of GNG11 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500256, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3:
Flow cytometric analysis of SH-SY5Y cells labeling GNG11. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA500256, 10ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.