Pancreatic triacylglycerol lipase Rabbit Polyclonal Antibody
cat.: HA500257
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 51 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Pancreatic triacylglycerol lipase aa 1-200 / 465. |
| Positive control: | Mouse pancreas tissue lysate, rat pancreas tissue lysate, rat pancreas tissue, A549, SiHa. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:20,000 1:50-1:200 1:100-1:500 1:500-1:1,000 |
| Uniprot #: | SwissProt: P16233 Human | Q6P8U6 Mouse | P27657 Rat |
| Alternative names: | lipase, pancreatic LIPP_HUMAN Pancreatic lipase Pancreatic triacylglycerol lipase PL PNLIP PNLIPD PTL Triacylglycerol acylhydrolase |
Images
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Fig1:
Western blot analysis of Pancreatic triacylglycerol lipase on different lysates with Rabbit anti-Pancreatic triacylglycerol lipase antibody (HA500257) at 1/20,000 dilution. Lane 1: Mouse pancreas tissue lysate Lane 2: Rat pancreas tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 51 kDa Observed band size: 45/51 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500257) at 1/20,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: Immunohistochemical analysis of paraffin-embedded rat pancreas tissue using anti-Pancreatic triacylglycerol lipase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500257, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Flow cytometric analysis of Pancreatic triacylglycerol lipase was done on A549 cells. The cells were fixed, permeabilized and stained with the primary antibody (HA500257, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig4: ICC staining of Pancreatic triacylglycerol lipase in SiHa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500257, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.