Elongation factor 1-gamma Rabbit Polyclonal Antibody
cat.: HA500258
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 50/56 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human EEF1G aa 10-60 / 437. |
| Positive control: | HeLa cell lysate, MCF7 cell lysate, NIH/3T3 cell lysate, mouse testis tissue lysate, mouse thymus tissue lysate, rat testis tissue lysate, mouse cerebellum tissue, human esophagus tissue, rat pancreas tissue. |
| Subcellular location: | cytosol, extracellular exosome, nucleus, cytoplasm, membrane. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:1,000 1:200-1:1800 |
| Uniprot #: | SwissProt: P26641 Human | Q9D8N0 Mouse | Q68FR6 Rat |
| Alternative names: | 2610301D06Rik AA407312 eEF 1B gamma EEF 1G eEF-1B gamma EEF1G EF 1 gamma EF 1G EF-1-gamma EF1 gamma EF1G EF1G_HUMAN Elongation factor 1 gamma Elongation factor 1-gamma Eukaryotic translation elongation factor 1 gamma GIG 35 GIG35 MGC103354 MGC114210 MGC144723 MGC144724 MGC94929 Pancreatic tumor related protein PRO1608 Translation elongation factor eEF 1 gamma chain |
Images
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Fig1:
Western blot analysis of Elongation factor 1-gamma on different lysates with Rabbit anti-Elongation factor 1-gamma antibody (HA500258) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: MCF7 cell lysate (20 µg/Lane) Lane 3: NIH/3T3 cell lysate (20 µg/Lane) Lane 4: Mouse testis tissue lysate (40 µg/Lane) Lane 5: Mouse thymus tissue lysate (40 µg/Lane) Lane 6: Rat testis tissue lysate (40 µg/Lane) Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500258) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-EEF1G antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 90) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500258, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human esophagus tissue using anti-EEF1G antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500258, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded rat pancreas tissue using anti-EEF1G antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500258, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.