TMEM119 Rabbit Polyclonal Antibody
cat.: HA500260
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 29 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human TMEM119 aa 11-52 / 283. |
| Positive control: | Mouse brain tissue lysate, mouse hippocampus tissue lysate, SH-SY5Y. |
| Subcellular location: | Endoplasmic reticulum membrane, Cell membrane, Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell FC |
1:5,000 1:50-1:100 1:100-1:500 |
| Uniprot #: | SwissProt: Q4V9L6 Human | Q8R138 Mouse |
| Alternative names: | OBIF Osteoblast induction factor PSEC0199 Transmembrane protein 119 UNQ731/PRO1415 |
Images
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Fig1:
Western blot analysis of TMEM119 on different lysates with Rabbit anti-TMEM119 antibody (HA500260) at 1/5,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Mouse hippocampus tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 29 kDa Observed band size: 45 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500260) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining of TMEM119 in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500260, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3: Flow cytometric analysis of TMEM119 was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (HA500260, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.