FARS2 Rabbit Polyclonal Antibody
cat.: HA500261
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 52 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human FARS2 aa 50-100 / 451.
Positive control: Rat colon tissue lysate, 293T cell lysate, SH-SY5Y, rat testis tissue, human small intestine tissue, mouse cerebellum tissue, SiHa.
Subcellular location: Mitochondrion matrix, Mitochondrion.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
1:1,000
1:50
1:100
Uniprot #: SwissProt: O95363 Human | Q99M01 Mouse | Q6AYQ3 Rat
Alternative names: dJ236A3.1 (phenylalanine tRNA synthetase) dJ520B18.2 (FARS1 (phenylalanine tRNA synthetase)) FARS1 Fars2 HSPC320 Phenylalanine translase Phenylalanine tRNA ligase 2, mitochondrial Phenylalanine tRNA ligase Phenylalanine tRNA synthetase 1 (mitochondrial) Phenylalanine tRNA synthetase 2 (mitochondrial) Phenylalanine--tRNA ligase Phenylalanyl tRNA synthetase 2 Phenylalanyl-tRNA synthetase, mitochondrial PheRS SYFM_HUMAN
Images
HA500261_1.jpg Fig1: Western blot analysis of FARS2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500261, 1/1,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Rat colon tissue lysate
Lane 2: 293T cell lysate

Predicted band size: 52 kDa
Observed band size: 52 kDa
HA500261_2.jpg Fig2: ICC staining of FARS2 in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500261, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
HA500261_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-FARS2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500261, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500261_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-FARS2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500261, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500261_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-FARS2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500261, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500261_6.jpg Fig6: ICC staining of FARS2 in SiHa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500261, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.