BRG1 Rabbit Polyclonal Antibody
cat.: HA500262
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 181/187 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human BRG1 aa 200-300 / 1647. |
| Positive control: | 293 cell lysate, Daudi cell lysate, mouse testis tissue, human lung carcinoma tissue, Wild-type HepG2 whole cell lysate. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:50-1:200 |
| Uniprot #: | SwissProt: P51532 Human | Q3TKT4 Mouse |
| Alternative names: | ATP dependent helicase SMARCA4 ATP-dependent helicase SMARCA4 BAF 190 BAF190 BAF190A Brahma protein like 1 BRG1 BRG1 associated factor 190A BRG1 protein BRG1-associated factor 190A BRM/SWI2 related gene 1 Global transcription activator homologous sequence global transcription activator snf2l4 Homeotic gene regulator hSNF2b Mitotic growth and transcription activator MRD16 Nuclear protein GRB1 Protein brahma homolog 1 Protein BRG-1 Protein BRG1 RTPS2 SMARC A4 SMARCA4 SMCA4_HUMAN SNF2 SNF2 beta SNF2 like 4 SNF2-beta SNF2B SNF2L4 SNF2LB Sucrose nonfermenting like 4 SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily A member 4 SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4 SWI2 Transcription activator BRG1 |
Images
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Fig1:
Western blot analysis of BRG1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500262, 1/1,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: 293 cell lysate Lane 2: Daudi cell lysate |
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Fig2:
All lanes: Western blot analysis of BRG1 with anti-BRG1 antibody (HA500262) at 1:500 dilution. Lane 1: Wild-type HepG2 whole cell lysate (10 µg). Lane 2: BRG1 knockdown HepG2 whole cell lysate (10 µg). HA500262 was shown to specifically react with BRG1 in wild-type HepG2 cells. Weakened band was observed when BRG1 knockdown sample was tested. Wild-type and BRG1 knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (HA500262, 1:500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig3: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-BRG1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500262, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-BRG1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500262, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.