PDE6 alpha Rabbit Polyclonal Antibody
cat.: HA500264
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 100 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human PDE6 alpha aa 201-400. |
| Positive control: | Rat eyeballs tissue lysate, K562 cell lysate, 293T cell lysate, rat kidney tissue lysate, Hela, rat eyeball tissue, mouse eyeball tissue. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:500 1:200 1:600 |
| Uniprot #: | SwissProt: P16499 Human | P27664 Mouse Entrez Gene: 307401 Rat |
| Alternative names: | 5''-cyclic phosphodiesterase subunit alpha CGPR A GMP PDE alpha GMP-PDE alpha PDE 6 alpha PDE 6A PDE V B1 PDE V-B1 PDE6A PDE6A_HUMAN PDEA Phosphodiesterase 6 alpha Phosphodiesterase 6A alpha subunit Phosphodiesterase 6A Phosphodiesterase 6A cGMP specific rod alpha Retinal Rod Photoreceptor cGMP Phosphodiesterase alpha Rod cGMP specific 3' 5' cyclic phosphodiesterase alpha subunit Rod cGMP-specific 3'' |
Images
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Fig1:
Western blot analysis of PDE6 alpha on different lysates with Rabbit anti-PDE6 alpha antibody (HA500264) at 1/1,000 dilution. Lane 1: Mouse eyeball Lane 2: Mouse kidney Lane 3: Rat eyeball Lysates/proteins at 20 µg/Lane. Exposure time: 6 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500264, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 99.5 kDa Observed band size: 100 kDa |
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Fig2: ICC staining of PDE6 alpha in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500264, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3: Immunohistochemical analysis of paraffin-embedded rat eyeball tissue using anti-PDE6 alpha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500264, 1/600) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded mouse eyeball tissue using anti-PDE6 alpha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500264, 1/600) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.