CCL2 Rabbit Polyclonal Antibody
cat.: HA500267
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Human |
| Applications: | WB, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 11 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human CCL2 aa 30-70 / 99. |
| Positive control: | Mouse testis tissue lysates. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB FC |
1:500-1:1,000 1:500-1:1,000 |
| Uniprot #: | SwissProt: P13500 Human | P10148 Mouse |
| Alternative names: | C-C motif chemokine 2 CCL2 CCL2_HUMAN Chemokine (C C motif) ligand 2 GDCF 2 GDCF-2 GDCF2 HC11 HSMCR30 JE MCAF MCP 1 MCP-1 MCP1 MGC9434 Monocyte chemoattractant protein 1 Monocyte chemotactic and activating factor Monocyte chemotactic protein 1 Monocyte secretory protein JE SCYA2 Small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig je) Small inducible cytokine A2 Small inducible cytokine subfamily A (Cys Cys), member 2 Small-inducible cytokine A2 SMC CF SMC-CF SMCCF |
Images
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Fig1: Western blot analysis of CCL2 on mouse testis tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500267, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Flow cytometric analysis of CCL2 was done on A549 cells. The cells were fixed, permeabilized and stained with the primary antibody (HA500267, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.