CD116 Rabbit Polyclonal Antibody
cat.: HA500268
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 46 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human CD116 aa 351-400 (Cytoplasmic domain). |
| Positive control: | Hela cell lysate, Jurkat cell lysate, 293T cell lysate, HepG2 cell lysate, human skin tissue, SiHa, A431, Hela. |
| Subcellular location: | Cell membrane; Secreted. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:1,000 1:200-1:400 1:1,000 1:500-1:1,000 |
| Uniprot #: | SwissProt: P15509 Human |
| Alternative names: | CD_antigen=CD116 CD116 CD116 antigen CDw116 Colony stimulating factor 2 receptor alpha chain Colony stimulating factor 2 receptor alpha low affinity Colony stimulating factor 2 receptor alpha subunit CSF 2R CSF2R CSF2R_HUMAN CSF2RA CSF2RAX CSF2RAY CSF2RX CSF2RY GM CSF R alpha GM CSF receptor alpha subunit GM-CSF-R-alpha GMCSFR GMCSFR-alpha GMR-alpha Granulocyte macrophage colony stimulating factor receptor alpha chain Granulocyte-macrophage colony-stimulating factor receptor subunit alpha SMDP4 |
Images
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Fig1:
Western blot analysis of CD116 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500268, 1/1,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: Jurkat cell lysate Lane 3: 293T cell lysate Lane 4: HepG2 cell lysate Predicted band size: 46 kDa Observed band size: 65/55 kDa (Glycosylation) |
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Fig2: Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-CD116 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500268, 1/1,000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunocytochemistry analysis of SiHa cells labeling CD116 with Rabbit anti-CD116 antibody (HA500268) at 1/400 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CD116 antibody (HA500268) at 1/400 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig4: ICC staining of CD116 in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500268, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig5: ICC staining of CD116 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500268, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig6:
Flow cytometric analysis of Hela cells labeling CD116. Cells were fixed and permeabilized. Then stained with the primary antibody (HA500268, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.