IL-2RG Rabbit Polyclonal Antibody
cat.: HA500269
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 42 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human IL-2RG aa 301-350. |
| Positive control: | Mouse spleen tissue lysate, rat thymus tissue lysate, SH-SY5Y, SiHa, human tonsil tissue, human spleen tissue. |
| Subcellular location: | Cell membrane, Cell surface. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:1,000 1:200 1:600 |
| Uniprot #: | SwissProt: P31785 Human | P34902 Mouse Entrez Gene: 140924 Rat |
| Alternative names: | CD132 CD132 antigen CIDX common cytokine receptor gamma chain Cytokine receptor common subunit gamma Gamma C gamma(c) gammaC IL-2 receptor subunit gamma IL-2R gamma chain IL-2R subunit gamma IL-2RG Il2rg IL2RG_HUMAN IMD4 interleukin 2 receptor, gamma Interleukin-2 receptor subunit gamma p64 SCIDX SCIDX1 |
Images
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Fig1:
Western blot analysis of IL-2RG on different lysates with Rabbit/Mouse anti-IL-2RG antibody (HA500269) at 1/1,000 dilution. Lane 1: Mouse spleen tissue lysate Lane 2: Rat thymus tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 42 kDa Observed band size: 55 kDa (Glycoprotein) Exposure time: 3 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500269) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of SH-SY5Y cells labeling IL-2RG with with Rabbit anti-IL-2RG antibody (HA500269) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 30minutes, permeabilized with 0.01% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-IL-2RG antibody (HA500269) at 1/200 dilution in 2% BSA overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. |
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Fig3:
Immunocytochemistry analysis of SiHa cells labeling IL-2RG with with Rabbit anti-IL-2RG antibody (HA500269) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 30minutes, permeabilized with 0.01% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-IL-2RG antibody (HA500269) at 1/200 dilution in 2% BSA overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-IL-2RG antibody (HA500269) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500269) at 1/600 dilution for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-IL-2RG antibody (HA500269) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500269) at 1/600 dilution for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.