LEF1 Rabbit Polyclonal Antibody
cat.: HA500273
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, IF-Cell
Clonality: Polyclonal
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 44 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human LEF1 aa 150-200 / 399.
Positive control: SW480 (Human colorectal zdenocarcinoma cell) cell lysates, human tonsil tissue, mouse colon tissue, mouse spleen tissue, rat liver tissue, HT-29.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  IHC-P
  IF-Cell
1:500-1:2,000
1:100-1:500
1:50-1:100
Uniprot #: SwissProt: Q9UJU2 Human | P27782 Mouse | Q9QXN1 Rat
Alternative names: DKFZp586H0919 FLJ46390 LEF 1 LEF-1 Lef1 LEF1_HUMAN Lymphoid enhancer binding factor 1 Lymphoid enhancer-binding factor 1 T cell specific transcription factor 1 alpha T cell-specific transcription factor 1-alpha TCF 1 alpha TCF1 alpha TCF1-alpha TCF10 TCF1alpha TCF7L3 Transcription factor T cell specific 1 alpha
Images
HA500273_1.jpg Fig1: Western blot analysis of LEF1 on SW480 (Human colorectal zdenocarcinoma cell) cell lysate with Rabbit anti-LEF1 antibody (HA500273) at 1/1,000 dilution.

Lysates/proteins at 20 µg/Lane.
Exposure time: 3 minutes 10 seconds; ECL: K1801

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA500273, 1/1,000 in 5% NFDM/TBST, 2 hours at room temperature
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 44 kDa
Observed band size: 55 kDa
HA500273_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-LEF1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500273, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500273_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-LEF1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500273, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500273_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-LEF1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500273, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500273_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-LEF1 antibody (HA500273) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500273) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500273_6.jpg Fig6: ICC staining of LEF1 in HT-29 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500273, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.