Product Type: | Rabbit polyclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | WB |
Clonality: | Polyclonal |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Immunogen affinity purified. |
Molecular weight: | Predicted band size: 43 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within human MVD aa 350-400 / 400. |
Positive control: | HEK-293 cell lysate, HepG2 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, rat brain tissue lysate, human liver tissue, human lung tissue. |
Subcellular location: | Cytoplasm. |
Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:100-1:500 |
Uniprot #: | SwissProt: P53602 Human | Q99JF5 Mouse | Q62967 Rat |
Alternative names: | Diphosphomevalonate decarboxylase FP17780 MDDase Mevalonate (diphospho) decarboxylase Mevalonate (diphospho)decarboxylase Mevalonate pyrophosphate decarboxylase MPD Mvd MVD1_HUMAN |
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Fig1:
Western blot analysis of MVD on different lysates with Rabbit anti-MVD antibody (HA500275) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: HepG2 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: C6 cell lysate Lane 5: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 43 kDa Observed band size: 45 kDa Exposure time: 51 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500275) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |