MVD Rabbit Polyclonal Antibody
cat.: HA500275
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 43 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human MVD aa 350-400 / 400.
Positive control: HEK-293 cell lysate, HepG2 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, rat brain tissue lysate, human liver tissue, human lung tissue.
Subcellular location: Cytoplasm.
Recommended Dilutions:
  WB
  IHC-P

1:500-1:2,000
1:100-1:500
Uniprot #: SwissProt: P53602 Human | Q99JF5 Mouse | Q62967 Rat
Alternative names: Diphosphomevalonate decarboxylase FP17780 MDDase Mevalonate (diphospho) decarboxylase Mevalonate (diphospho)decarboxylase Mevalonate pyrophosphate decarboxylase MPD Mvd MVD1_HUMAN
Images
HA500275_1.jpg Fig1: Western blot analysis of MVD on different lysates with Rabbit anti-MVD antibody (HA500275) at 1/1,000 dilution.

Lane 1: HEK-293 cell lysate
Lane 2: HepG2 cell lysate
Lane 3: NIH/3T3 cell lysate
Lane 4: C6 cell lysate
Lane 5: Rat brain tissue lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 43 kDa
Observed band size: 45 kDa

Exposure time: 51 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500275) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.