PP-X Rabbit Polyclonal Antibody
cat.: HA500276
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 35 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human PP-X aa 151-307 / 307. |
| Positive control: | 293T cell lysates, human colon tissue. |
| Subcellular location: | Centrosome, Nucleus, Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:100-1:500 |
| Uniprot #: | SwissProt: P60510 Human |
| Alternative names: | PP X PP-X Pp4 PP4C PP4C_HUMAN PPH3 PPP4 ppp4c PPX protein phosphatase 4 (formerly X), catalytic subunit Protein phosphatase 4 catalytic subunit Protein phosphatase X protein phosphatase X, catalytic subunit Serine/threonine protein phosphatase 4 catalytic subunit Serine/threonine-protein phosphatase 4 catalytic subunit |
Images
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Fig1: Western blot analysis of PP-X on 293T cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500276, 1/1,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-PP-X antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500276, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.