TEF1 Rabbit Polyclonal Antibody
cat.: HA500277
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 48 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human TEF1 aa 1-150 / 426. |
| Positive control: | Mouse liver tissue lysate, mouse skeletal muscle tissue lysate, rat skeletal muscle tissue, human fetal skeletal muscle tissue, Hela. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P FC |
1:500 1:400 1:500-1:1,000 |
| Uniprot #: | SwissProt: P28347 Human | P30051 Mouse |
| Alternative names: | AA Atrophia areata peripapillary chorioretinal degeneration NTEF 1 NTEF-1 NTEF1 Protein GT IIC Protein GT-IIC REF 1 REF1 SV40 transcriptional enhancer factor TCF 13 TCF-13 TCF13 TEA domain family member TEA domain family member 1 (SV40 transcriptional enhancer factor) TEA domain family member 1 TEAD 1 TEAD 1 protein TEAD-1 TEAD1 TEAD1 protein TEAD1_HUMAN TEF 1 TEF1 Transcription factor 13 (SV40 transcriptional enhancer factor) Transcription factor 13 Transcriptional enhancer factor 1 Transcriptional enhancer factor TEF-1 Transcriptional enhancer factor TEF1 |
Images
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Fig1:
Western blot analysis of TEF1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500277, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse liver tissue lysate Lane 2: Mouse skeletal muscle tissue lysate Predicted band size: 48 kDa Observed band size: 43 kDa |
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Fig2: Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-TEF1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500277, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-TEF1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500277, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Flow cytometric analysis of TEF1 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (HA500277, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.