CCR3 Rabbit Polyclonal Antibody
cat.: HA500279
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 41 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human CCR3 aa 1-34 / 355. |
| Positive control: | K562 cell lysate, Jurkat cell lysate, HepG2 cell lysate, SW480. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IF-Cell |
1:500 1:50-1:200 |
| Uniprot #: | SwissProt: P51677 Human |
| Alternative names: | B chemokine receptor b-chemokine receptor C C chemokine receptor type 3 C C CKR 3 C-C chemokine receptor type 3 C-C CKR-3 CC chemokine receptor 3 CC chemokine receptor type 3 CC CKR 3 CC R3 CC-CKR-3 CCCKR3 CCR 3 CCR-3 CCR3 CCR3_HUMAN CD 193 CD193 CD193 antigen Chemokine (C C motif) receptor 3 chemokine (C-C motif) receptor 3 Chemokine (CC) receptor 1 like 2 Chemokine CC motif receptor 3 Chemokine receptor 3 CK R3 CKR 3 CKR3 CMKB R3 CMKBR 3 CMKBR1L2 CMKBR3 Eosinophil CC chemokine receptor 3 Eosinophil eotaxin receptor Eotaxin receptor Macrophage inflammatory protein 1 alpha receptor like 2 MGC102841 MIP1 Alpha RL2 |
Images
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Fig1:
Western blot analysis of CCR3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500279, 1/1,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: K562 cell lysate Lane 2: Jurkat cell lysate Lane 3: HepG2 cell lysate Predicted band size: 41 kDa Observed band size: 60 kDa |
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Fig2: ICC staining of CCR3 in SW480 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500279, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.