Lck Rabbit Polyclonal Antibody
cat.: HA500280
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 58 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Lck aa 100-300 / 509. |
| Positive control: | Rat thymus tissue lysate, Jurkat cell lysate, Hela. |
| Subcellular location: | Cytosol, Cell membrane. |
| Recommended Dilutions:
WB FC |
1:500-1:2,000 1:500-1:1,000 |
| Uniprot #: | SwissProt: P06239 Human | Q01621 Rat |
| Alternative names: | IMD22 LCK Lck p56 LCK proto-oncogene, Src family tyrosine kinase LCK_HUMAN Leukocyte C-terminal Src kinase LSK Lymphocyte cell specific protein tyrosine kinase Lymphocyte cell-specific protein-tyrosine kinase Lymphocyte specific protein tyrosine kinase Membrane associated protein tyrosine kinase Oncogene lck P56 LCK p56(LSTRA) protein tyrosine kinase p56-LCK p56lck pp58 lck pp58lck Protein YT16 Proto oncogene tyrosine protein kinase LCK Proto-oncogene Lck Protooncogene tyrosine protein kinase LCK T cell specific protein tyrosine kinase T cell-specific protein-tyrosine kinase T lymphocyte specific protein tyrosine kinase p56lck Tyrosine-protein kinase Lck YT 16 YT16 |
Images
|
Fig1:
Western blot analysis of Lck on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500280, 1/1,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Rat thymus tissue lysate Lane 2: Jurkat cell lysate |
|
Fig2: Flow cytometric analysis of Lck was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (HA500280, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.