Lck Rabbit Polyclonal Antibody
cat.: HA500280
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 58 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Lck aa 100-300 / 509. |
| Positive control: | Rat thymus tissue lysate, Jurkat cell lysate, Hela. |
| Subcellular location: | Cytosol, Cell membrane. |
| Recommended Dilutions:
WB FC |
1:500-1:2,000 1:500-1:1,000 |
| Uniprot #: | SwissProt: P06239 Human | Q01621 Rat |
| Alternative names: | IMD22 LCK Lck p56 LCK proto-oncogene, Src family tyrosine kinase LCK_HUMAN Leukocyte C-terminal Src kinase LSK Lymphocyte cell specific protein tyrosine kinase Lymphocyte cell-specific protein-tyrosine kinase Lymphocyte specific protein tyrosine kinase Membrane associated protein tyrosine kinase Oncogene lck P56 LCK p56(LSTRA) protein tyrosine kinase p56-LCK p56lck pp58 lck pp58lck Protein YT16 Proto oncogene tyrosine protein kinase LCK Proto-oncogene Lck Protooncogene tyrosine protein kinase LCK T cell specific protein tyrosine kinase T cell-specific protein-tyrosine kinase T lymphocyte specific protein tyrosine kinase p56lck Tyrosine-protein kinase Lck YT 16 YT16 |
Images
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Fig1: Flow cytometric analysis of Lck was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (HA500280, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig2:
Western blot analysis of Lck on different lysates with Rabbit anti-Lck antibody (HA500280) at 1/1,000 dilution. Lane 1: Rat spleen tissue lysate Lysates/proteins at 20 µg/Lane. Exposure time: 10 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500280, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 58 kDa Observed band size: 55 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.