PLK1 Rabbit Polyclonal Antibody
cat.: HA500281
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 68 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human PLK1 aa 1-50 / 603. |
| Positive control: | HeLa whole cell lysate, HeLa treated with 100nM Nocodazole for 24 hours whole cell lysate, HeLa treated with 2mM thymidine for 16 hours then treated with 100nM Nocodazole for 24 hours whole cell lysate, HT-29 whole cell lysate, HT-29 treated with 100nM Nocodazole for 24 hours whole cell lysate, HT-29 treated with 2mM thymidine for 16 hours then treated with 100nM Nocodazole for 24 hours whole cell lysate, HepG2 cell lysate, Hela cell lysate, mouse testis tissue lysate, Hela. |
| Subcellular location: | Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore, Nucleus. |
| Recommended Dilutions:
WB FC |
1:1,000 1:500-1:1,000 |
| Uniprot #: | SwissProt: P53350 Human | Q07832 Mouse |
| Alternative names: | Cell cycle regulated protein kinase PLK 1 PLK PLK-1 plk1 PLK1_HUMAN Polo like kinase 1 Polo-like kinase 1 Serine/threonine protein kinase 13 Serine/threonine protein kinase PLK1 Serine/threonine-protein kinase 13 Serine/threonine-protein kinase PLK1 STPK 13 STPK13 |
Images
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Fig1:
Western blot analysis of PLK1 on different lysates with Rabbit anti-PLK1 antibody (HA500281) at 1/1,000 dilution. Lane 1: HeLa whole cell lysate Lane 2: HeLa treated with 100nM Nocodazole for 24 hours whole cell lysate Lane 3: HeLa treated with 2mM thymidine for 16 hours then treated with 100nM Nocodazole for 24 hours whole cell lysate Lane 4: HT-29 whole cell lysate Lane 5: HT-29 treated with 100nM Nocodazole for 24 hours whole cell lysate Lane 6: HT-29 treated with 2mM thymidine for 16 hours then treated with 100nM Nocodazole for 24 hours whole cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 68 kDa Observed band size: 68 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500281) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of PLK1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500281, 1/1,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HepG2 cell lysate Lane 2: Hela cell lysate Lane 3: Mouse testis tissue lysate Predicted band size: 68 kDa Observed band size: 68/55/40 kDa |
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Fig3: Flow cytometric analysis of PLK1 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (HA500281, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.