Triosephosphate isomerase Rabbit Polyclonal Antibody
cat.: HA500283
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 27 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Triosephosphate isomerase aa 1-200 / 249. |
| Positive control: | Rat brain tissue lysate, 293 cell lysate, HepG2 cell lysate, rat skeletal muscle tissue lysate, mouse skeletal muscle tissue lysate, mouse lung tissue lysate, human colon tissue, mouse liver tissue, 293T. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IHC-P FC |
1:1,000 1:400 1:500-1:1,000 |
| Uniprot #: | SwissProt: P60174 Human | P17751 Mouse | P48500 Rat |
| Alternative names: | epididymis secretory protein Li 49 HEL-S-49 MGC88108 TIM TPI 1 TPI TPI1 TPID Triose phosphate isomerase 1 Triose phosphate isomerase Triosephosphate isomerase 1 Triosephosphate isomerase |
Images
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Fig1:
Western blot analysis of Triosephosphate isomerase on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500283, 1/1,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Rat brain tissue lysate Lane 2: 293 cell lysate Lane 3: HepG2 cell lysate Lane 4: Rat skeletal muscle tissue lysate Lane 5: Mouse skeletal muscle tissue lysate Lane 6: Mouse lung tissue lysate Predicted band size: 27 kDa Observed band size: 27 kDa |
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Fig2: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Triosephosphate isomerase antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500283, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Triosephosphate isomerase antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500283, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Flow cytometric analysis of Triosephosphate isomerase was done on 293T cells. The cells were fixed, permeabilized and stained with the primary antibody (HA500283, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.