IFNAR2 Rabbit Polyclonal Antibody
cat.: HA500284
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 58 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human IFNAR2 aa 194-243 (Extracellular domain). |
| Positive control: | SK-Br-3 cell lysate, MCF-7 cell lysate, HepG2 cell lysate, human prostate tissue, SH-SY5Y, SiHa. |
| Subcellular location: | Cell membrane; Secreted. |
| Recommended Dilutions:
WB IHC-P IF-Cell |
1:500 1:600 1:200 |
| Uniprot #: | SwissProt: P48551 Human |
| Alternative names: | Human interferon alpha/beta receptor IFN alpha REC IFN R IFN-a/ßR2 IFN-a/ßRß IFN-alpha binding protein IFN-alpha/beta receptor 2 IFN-R-2 IFNABR IFNAR2 IFNARB IFNR INAR2_HUMAN Interferon (alpha beta and omega) receptor 2 Interferon alpha binding protein Interferon alpha/beta receptor 2 Interferon alpha/beta receptor beta chain Type I interferon receptor 2 Type I interferon receptor |
Images
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Fig1:
Western blot analysis of IFNAR2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500284, 1/1,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SK-Br-3 cell lysate Lane 2: MCF-7 cell lysate Lane 3: HepG2 cell lysate Predicted band size: 58 kDa Observed band size: 58 kDa |
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Fig2: Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-IFNAR2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500284, 1/600) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: ICC staining of IFNAR2 in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500284, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig4: ICC staining of IFNAR2 in SiHa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500284, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.