HNF1 alpha Rabbit Polyclonal Antibody
cat.: HA500285
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 67 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human HNF1 alpha aa 100-300 / 631. |
| Positive control: | HepG2 cell lysate, mouse uterus tissue lysate, human kidney tissue lysate, rat kidney tissue, human small intestine tissue, mouse liver tissue, HepG2. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P FC |
1:1,000-1:5,000 1:100-1:500 1:500-1:1,000 |
| Uniprot #: | SwissProt: P20823 Human | P22361 Mouse | P15257 Rat |
| Alternative names: | Albumin proximal factor Hepatic nuclear factor 1 alpha Hepatic nuclear factor 1 Hepatic transcription factor 1 alpha Hepatic transcription factor 1 Hepatocyte nuclear factor 1-alpha HNF 1 HNF 1A HNF-1-alpha HNF-1A hnf1a HNF1A_HUMAN Interferon production regulator factor LF B1 LF B1 hepatic nuclear factor LFB 1 LFB1 LFB1 hepatic nuclear factor Liver specific transcription factor LF B1 Liver specific transcription factor LFB1 Liver-specific transcription factor LF-B1 Maturity onset diabetes of the young 3 MODY 3 MODY3 TCF 1 TCF-1 TCF1 Transcription factor 1 Transcription factor 1 hepatic |
Images
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Fig1:
Western blot analysis of HNF1 alpha on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500285, 1/5,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HepG2 cell lysate Lane 2: Mouse uterus tissue lysate Lane 3: Human kidney tissue lysate |
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Fig2: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-HNF1 alpha antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500285, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-HNF1 alpha antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500285, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-HNF1 alpha antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500285, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Flow cytometric analysis of HepG2 cells labeling HNF1 alpha. Cells were fixed and permeabilized. Then stained with the primary antibody (HA500285, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.