IL-20R1 Rabbit Polyclonal Antibody
cat.: HA500286
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 62 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human IL-20R1 aa 471-520. |
| Positive control: | MCF-7 cell lysate, SH-SY5Y cell lysate, SH-SY5Y, human kidney tissue. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:500-1:2,000 1:200 1:200 |
| Uniprot #: | SwissProt: Q9UHF4 Human |
| Alternative names: | class II cytokine receptor ZCYTOR7 CRF2 8 CRF2-8 Cytokine receptor class II member 8 Cytokine receptor class-II member 8 Cytokine receptor family 2 member 8 I20RA_HUMAN IL 20R alpha IL 20R1 IL-20 receptor subunit alpha IL-20R-alpha IL-20R1 IL-20RA IL20 Receptor alpha IL20RA Interleukin 20 receptor alpha chain Interleukin 20 receptor, alpha interleukin-20 receptor I Interleukin-20 receptor subunit alpha ZcytoR7 |
Images
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Fig1:
Western blot analysis of IL-20R1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500286, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: MCF-7 cell lysate Lane 2: SH-SY5Y cell lysate Predicted band size: 62 kDa Observed band size: 67 kDa (Glycosylation) |
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Fig2: ICC staining of IL-20R1 in SH-SY5Y cells (green). Methanol fixed cells were blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500286, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-IL-20R1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500286, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.