GABRA2 Rabbit Polyclonal Antibody
cat.: HA500288
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 51 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human GABRA2 aa 29-200 / 451 (Extracellular). |
| Positive control: | Rat brain tissue lysates, mouse brain tissue lysate, mouse cerebellum tissue lysate, human brain tissue lysate, Hela. |
| Subcellular location: | Postsynaptic cell membrane, Cell membrane, Cytoplasmic vesicle membrane, Dendrite. |
| Recommended Dilutions:
WB FC |
1:500-1:20,000 1:500-1:1,000 |
| Uniprot #: | SwissProt: P47869 Human | P26048 Mouse | P23576 Rat |
| Alternative names: | GABA A receptor subunit alpha 2 GABA GABA(A) receptor subunit alpha 2 GABA(A) receptor subunit alpha-2 GABR A2 GABRA 2 GABRA2 GABRA2 protein Gamma aminobutyric acid (GABA) A receptor alpha 2 Gamma aminobutyric acid A receptor alpha 2 Gamma aminobutyric acid receptor alpha 2 subunit Gamma aminobutyric acid receptor subunit alpha 2 Gamma-aminobutyric acid receptor subunit alpha-2 GBRA2_HUMAN |
Images
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Fig1:
Western blot analysis of GABRA2 on different lysates with Rabbit anti-GABRA2 antibody (HA500288) at 1/20,000 dilution. Lane 1: Mouse brain(Unboiled) tissue lysate Lane 2: Mouse lung(Unboiled) tissue lysate(negative) Lane 3: Rat brain(Unboiled) tissue lysate Lane 4: Rat lung(Unboiled) tissue lysate(negative) Lysates/proteins at 20 µg/Lane. Exposure time: 59 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500288, 1/20,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 51 kDa Observed band size: 51 kDa |
|
Fig2:
Flow cytometric analysis of Hela cells labeling GABRA2. Cells were fixed and permeabilized. Then stained with the primary antibody (HA500288, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.