GABRB2 Rabbit Polyclonal Antibody
cat.: HA500289
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 59 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human GABRB2 aa 350-400 / 512 ((Cytoplasmic). |
| Positive control: | Human brian tissue lysate, rat cerebellum tissue lysate, rat brian tissue lysate, mouse brain tissue, A549. |
| Subcellular location: | Cytoplasmic. |
| Recommended Dilutions:
WB IHC-P FC |
1:1,000 1:200 1:500-1:1,000 |
| Uniprot #: | SwissProt: P47870 Human | P63137 Mouse | P63138 Rat |
| Alternative names: | GABA GABA(A) receptor beta 2 GABA(A) receptor subunit beta-2 GABA-A receptor, beta-2 polypeptide GABRB2 Gamma aminobutyric acid (GABA) A receptor, beta 2 Gamma aminobutyric acid A receptor beta 2 Gamma-aminobutyric acid receptor subunit beta-2 Gamma-aminobutyric-acid receptor subunit beta-2 GBRB2_HUMAN |
Images
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Fig1: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-GABRB2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500289, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig2: Flow cytometric analysis of GABRB2 was done on A549 cells. The cells were fixed, permeabilized and stained with the primary antibody (HA500289, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
|
Fig3:
Western blot analysis of GABRB2 on different lysates with Rabbit anti-GABRB2 antibody (HA500289) at 1/5,000 dilution. Lane 1: SH-SY5Y (Human neuroblastoma cell) cell lysate Lane 2: Mouse brain tissue lysate Lane 3: Rat brain tissue lysate Lysates/proteins at 15 µg/Lane. Exposure time: 3 minutes; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500289, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 59kDa Observed band size: 50-80 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.