IL-21R Rabbit Polyclonal Antibody
cat.: HA500290
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 59 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human IL-21R aa 51-100. |
| Positive control: | Rat spleen tissue lysate, rat liver tissue lysate, rat thymus tissue lysate, mouse spleen tissue lysate, mouse thymus tissue lysate, Daudi cell lysate, human spleen tissue. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB IHC-P |
1:500 1:600 |
| Uniprot #: | SwissProt: Q9HBE5 Human | Q9JHX3 Mouse Entrez Gene: 308977 Rat |
| Alternative names: | CD360 IL 21R IL-21 receptor IL-21R IL21 receptor IL21R IL21R_HUMAN Interleukin 21 receptor Interleukin-21 receptor MGC10967 NILR Novel interleukin receptor |
Images
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Fig1:
Western blot analysis of IL-21R on different lysates with rabbit anti-IL-21R antibody (HA500290) at 1/500 dilution. Lane 1: Rat spleen tissue lysate Lane 2: Rat liver tissue lysate Lane 3: Rat thymus tissue lysate Lysates/proteins at 10 µg per lane. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500290, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Predicted band size: 59 kDa Observed band size: 80 kDa (Glycosylation) |
|
Fig2:
Western blot analysis of IL-21R on different lysates with rabbit anti-IL-21R antibody (HA500290) at 1/500 dilution. Lane 1: Mouse spleen tissue lysate Lane 2: Mouse thymus tissue lysate Lane 3: Daudi cell lysate Lysates/proteins at 10 µg per lane. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500290, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Predicted band size: 59 kDa Observed band size: 80 kDa (Glycosylation) |
|
Fig3: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-IL-21R antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500290, 1/600) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.