IL-3RA Rabbit Polyclonal Antibody
cat.: HA500291
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 43 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human IL3RA aa 231-280. |
| Positive control: | MCF-7 cell lysates, Hela, human fallopian tube tissue, A549. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500 1:200 1:600 1:500-1:1,000 |
| Uniprot #: | SwissProt: P26951 Human |
| Alternative names: | CD123 CD123 antigen hIL 3Ra hIL-3Ra hIL3Ra IL 3 receptor alpha SP2 isoform IL 3R alpha IL-3 receptor subunit alpha IL-3R subunit alpha IL-3R-alpha IL-3RA IL3 Receptor alpha IL3R Il3ra IL3RA_HUMAN IL3RAX IL3RAY IL3RX IL3RY Interleukin 3 receptor alpha chain Interleukin 3 receptor, alpha (low affinity) Interleukin 3 receptor, alpha Interleukin-3 Receptor alpha Interleukin-3 receptor subunit alpha Interleukin3 receptor Interleukin3 receptor, Y-chromosomal |
Images
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Fig1:
Western blot analysis of IL-3RA on MCF-7 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500291, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Predicted band size: 43 kDa Observed band size: 45 kDa |
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Fig2: ICC staining of IL-3RA in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500291, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3: Immunohistochemical analysis of paraffin-embedded human fallopian tube tissue using anti-IL-3RA antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500291, 1/600) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Flow cytometric analysis of A549 cells labeling IL-3RA. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA500291, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.