GNAI2 Rabbit Polyclonal Antibody
cat.: HA500293
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 40/38//36/42/36/35 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human GNAI2 aa 50-150 / 355. |
| Positive control: | PC-12 cell lysates, 293 cell lysate, MCF-7 cell lysate, mouse hippocampus tissue lysate, MCF-7. |
| Subcellular location: | Cell membrane, Cytoplasm, Cytoskeleton, Membrane. |
| Recommended Dilutions:
WB FC |
1:500-1:2,000 1:100-1:500 |
| Uniprot #: | SwissProt: P04899 Human | P08752 Mouse | P04897 Rat |
| Alternative names: | Adenylate cyclase inhibiting G alpha protein Adenylate cyclase-inhibiting G alpha protein GIP GNAI 2 Gnai2 GNAI2_HUMAN GNAI2B GTP binding regulatory protein Gi alpha 2 chain Guanine nucleotide binding protein G protein alpha inhibiting activity polypeptide 2 Guanine nucleotide-binding protein G(i) subunit alpha-2 H LUCA15.1 H LUCA16.1 WUGSC:H LUCA15.1 WUGSC:H LUCA16.1 |
Images
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Fig1: Western blot analysis of GNAI2 on PC-12 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500293, 1/1,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of GNAI2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500293, 1/1,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: 293 cell lysate Lane 2: MCF-7 cell lysate Lane 3: Mouse hippocampus tissue lysate |
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Fig3: Flow cytometric analysis of GNAI2 was done on MCF-7 cells. The cells were fixed, permeabilized and stained with the primary antibody (HA500293, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.