GNAI2 Rabbit Polyclonal Antibody
cat.: HA500293
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 40 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human GNAI2 aa 50-150 / 355.
Positive control: PC-12 cell lysates, 293 cell lysate, MCF-7 cell lysate, mouse hippocampus tissue lysate, RAW264.7, PC-12, MCF-7.
Subcellular location: Cell membrane, Cytoplasm, Cytoskeleton, Membrane.
Recommended Dilutions:
  WB
  IF-Cell
  FC
1:1,000-1:2,000
1:100-1:200
1:100-1:500
Uniprot #: SwissProt: P04899 Human | P08752 Mouse | P04897 Rat
Alternative names: Adenylate cyclase inhibiting G alpha protein Adenylate cyclase-inhibiting G alpha protein GIP GNAI 2 Gnai2 GNAI2_HUMAN GNAI2B GTP binding regulatory protein Gi alpha 2 chain Guanine nucleotide binding protein G protein alpha inhibiting activity polypeptide 2 Guanine nucleotide-binding protein G(i) subunit alpha-2 H LUCA15.1 H LUCA16.1 WUGSC:H LUCA15.1 WUGSC:H LUCA16.1
Images
HA500293_1.jpg Fig1: Western blot analysis of GNAI2 on different lysates with Rabbit anti-GNAI2 antibody (HA500293) at 1/1,000 dilution.

Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-GNAI2 KD cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 40 kDa
Observed band size: 40 kDa

Exposure time: 10 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500293) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA500293_2.jpg Fig2: Western blot analysis of GNAI2 on PC-12 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500293, 1/1,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
HA500293_3.jpg Fig3: Western blot analysis of GNAI2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500293, 1/1,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: 293 cell lysate
Lane 2: MCF-7 cell lysate
Lane 3: Mouse hippocampus tissue lysate
HA500293_4.jpg Fig4: Immunocytochemistry analysis of RAW264.7 cells labeling GNAI2 with Rabbit anti-GNAI2 antibody (HA500293) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-GNAI2 antibody (HA500293) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
HA500293_5.jpg Fig5: Immunocytochemistry analysis of PC-12 cells labeling GNAI2 with Rabbit anti-GNAI2 antibody (HA500293) at 1/200 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-GNAI2 antibody (HA500293) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
HA500293_6.jpg Fig6: Flow cytometric analysis of GNAI2 was done on MCF-7 cells. The cells were fixed, permeabilized and stained with the primary antibody (HA500293, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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