GNAI2 Rabbit Polyclonal Antibody
cat.: HA500293
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 40 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human GNAI2 aa 50-150 / 355. |
| Positive control: | PC-12 cell lysates, 293 cell lysate, MCF-7 cell lysate, mouse hippocampus tissue lysate, RAW264.7, PC-12. |
| Subcellular location: | Cell membrane, Cytoplasm, Cytoskeleton, Membrane. |
| Recommended Dilutions:
WB IF-Cell |
1:1,000-1:2,000 1:100-1:200 |
| Uniprot #: | SwissProt: P04899 Human | P08752 Mouse | P04897 Rat |
| Alternative names: | Adenylate cyclase inhibiting G alpha protein Adenylate cyclase-inhibiting G alpha protein GIP GNAI 2 Gnai2 GNAI2_HUMAN GNAI2B GTP binding regulatory protein Gi alpha 2 chain Guanine nucleotide binding protein G protein alpha inhibiting activity polypeptide 2 Guanine nucleotide-binding protein G(i) subunit alpha-2 H LUCA15.1 H LUCA16.1 WUGSC:H LUCA15.1 WUGSC:H LUCA16.1 |
Images
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Fig1:
Western blot analysis of GNAI2 on different lysates with Rabbit anti-GNAI2 antibody (HA500293) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-GNAI2 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 40 kDa Observed band size: 40 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500293) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of GNAI2 on different lysates with Rabbit anti-GNAI2 antibody (HA500293) at 1/2,000 dilution. Lane 1: U-937 (Human acute monocytic leukemia cell) cell lysate Lane 2: MCF-7 (Human breast cancer cell) cell lysate Lane 3: RAW264.7 (Mouse monocytic macrophage leukemia cell) cell lysate Lane 4: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate Lane 5: mouse brain tissue lysate Lane 6: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Exposure time: 4 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500293, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 40 kDa Observed band size: 40 kDa |
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Fig3:
Immunocytochemistry analysis of RAW264.7 cells labeling GNAI2 with Rabbit anti-GNAI2 antibody (HA500293) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-GNAI2 antibody (HA500293) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig4:
Immunocytochemistry analysis of PC-12 cells labeling GNAI2 with Rabbit anti-GNAI2 antibody (HA500293) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-GNAI2 antibody (HA500293) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.