NEDD1 Rabbit Polyclonal Antibody
cat.: HA500294
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 72 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human NEDD1 aa 1-200 / 660. |
| Positive control: | Mouse lung lysates, human skin tissue, SiHa. |
| Subcellular location: | Centrosome. |
| Recommended Dilutions:
WB IHC-P FC |
1:500-1:2,000 1:100-1:500 1:100-1:500 |
| Uniprot #: | SwissProt: Q8NHV4 Human | P33215 Mouse |
| Alternative names: | FLJ35902 GCP WD NEDD-1 NEDD1 NEDD1_HUMAN Neural precursor cell expressed developmentally down-regulated protein 1 Neural precursor cell expressed, developmentally down regulated gene 1 Protein NEDD1 TUBGCP7 |
Images
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Fig1: Western blot analysis of NEDD1 on mouse lung lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500294, 1/1,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-NEDD1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500294, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3: Flow cytometric analysis of NEDD1 was done on SiHa cells. The cells were fixed, permeabilized and stained with the primary antibody (HA500294, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.