| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 43 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Protor-1 aa 200-388 / 388. |
| Positive control: | MCF-7 cell lysate, Mouse liver tissue lysate. |
| Subcellular location: | Cytosol. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: P85299 Human | Q812A5 Mouse | Q5FVG6 Rat |
| Alternative names: | Arhgap8 AU043908 C030017C09Rik C78947 FLJ20185 FLJ20185k MGC109513 MGC32512 PP610 Proline rich 5 (renal) Proline-rich protein 5 Protein observed with Rictor-1 Protor-1 PROTOR1 PRR5 PRR5_HUMAN PTOR1 Rho GTPase activating protein 8 RP1-181C9.3 |
|
Fig1:
Western blot analysis of PRR5 on different lysates with Rabbit anti-PRR5 antibody (HA500295) at 1/2,000 dilution. Lane 1: MCF-7 cell lysate(20 µg/Lane) Lane 2: Mouse liver tissue lysate(40 µg/Lane) Exposure time: 110 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500295, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 43 kDa Observed band size: 43/41 kDa |