Myt1 Rabbit Polyclonal Antibody
cat.: HA500296
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 122 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Myt1 aa 900-1121 / 1121. |
| Positive control: | Hela cell lysate, 293T cell lysate, human seminal vesicle tissue, Hela. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P FC |
1:500-1:2,000 1:100-1:500 1:500-1:1,000 |
| Uniprot #: | SwissProt: Q01538 Human |
| Alternative names: | C20orf36 KIAA0835 KIAA1050 MTF1 Myelin transcription factor 1 Myelin transcription factor I MYT1 MYT1_HUMAN MyTI PLPB1 Proteolipid protein-binding protein |
Images
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Fig1:
Western blot analysis of Myt1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500296, 1/1,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: 293T cell lysate |
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Fig2: Immunohistochemical analysis of paraffin-embedded human seminal vesicle tissue using anti-Myt1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500296, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Flow cytometric analysis of Hela cells labeling Myt1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA500296, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.