HLA E Rabbit Polyclonal Antibody
cat.: HA500299
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 40 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human HLA E aa 22-200 / 358 (Extracellular). |
| Positive control: | SiHa, A549 cell lysate, A431 cell lysate. |
| Subcellular location: | Golgi apparatus membrane, Cell membrane, Secreted. |
| Recommended Dilutions:
WB FC |
1:1,000 1:100-1:500 |
| Uniprot #: | SwissProt: P13747 Human |
| Alternative names: | HLA class I histocompatibility antigen E alpha chain EA1.2 EA2.1 HLA 6.2 HLA class I histocompatibility antigen alpha chain E HLA class I histocompatibility antigen E alpha chain precursor HLA class I histocompatibility antigen E alpha chain precursor HLA6.2 HLAE Lymphocyte antigen Major histocompatibility complex class I E MHC MHC class I antigen E MHC HLA E alpha 1 MHC HLA E alpha 2.1 QA1 |
Images
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Fig1: Flow cytometric analysis of HLA E was done on SiHa cells. The cells were fixed, permeabilized and stained with the primary antibody (HA500299, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
|
Fig2:
Western blot analysis of HLA E on different lysates with Rabbit anti-HLA E antibody (HA500299) at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: A431 cell lysate Lysates/proteins at 30 µg/Lane. Exposure time: 3 minutes; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500299, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/100,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 40 kDa Observed band size: 40 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.