TAZ Rabbit Polyclonal Antibody
cat.: HA500300
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 44 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human TAZ aa 1-200 / 400. |
| Positive control: | Human placenta tissue lysate, 293T cell lysate, human kidney tissue, mouse brain tissue, A549, HT-29. |
| Subcellular location: | Cell membrane, Cytoplasm, Membrane, Nucleus. |
| Recommended Dilutions:
WB IHC-P IF-Cell |
1:500-1:1,000 1:100-1:500 1:100-1:200 |
| Uniprot #: | SwissProt: Q9GZV5 Human | Q9EPK5 Mouse |
| Alternative names: | DKFZP586I1419 FLJ27004 FLJ45718 OTTHUMP00000215994 OTTHUMP00000215995 OTTHUMP00000215996 OTTHUMP00000216001 TAZ Transcriptional co activator with PDZ binding motif Transcriptional coactivator with PDZ binding motif Transcriptional coactivator with PDZ-binding motif WW domain containing transcription regulator 1 WW domain containing transcription regulator protein 1 WW domain-containing transcription regulator protein 1 WWTR 1 WWTR1 WWTR1_HUMAN |
Images
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Fig1:
Western blot analysis of TAZ on different lysates with Rabbit anti-TAZ antibody (HA500300) at 1/5,000 dilution. Lane 1: HeLa Lane 2: PANC-1 Lane 3: SH-SY5Y Lane 4: SK-MEL-28 Lane 5: Raji Lane 6: A375 Lysates/proteins at 20 µg/Lane. Exposure time: 15 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500300, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 44.1 kDa Observed band size: 52 kDa |
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Fig2: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-TAZ antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500300, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-TAZ antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500300, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: ICC staining of TAZ in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500300, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig5: ICC staining of TAZ in HT-29 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500300, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.