Adipose Triglyceride Lipase Rabbit Polyclonal Antibody
cat.: HA500301
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 55 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Adipose Triglyceride Lipase aa 450-504 / 504 (Lumenal). |
| Positive control: | 293 cell lysate, HepG2 cell lysate, A431 cell lysate, human pancreas tissue. |
| Subcellular location: | Cell membrane, Lipid droplet. |
| Recommended Dilutions:
WB IHC-P |
1:1,000-1:2,000 1:100-1:500 |
| Uniprot #: | SwissProt: Q96AD5 Human |
| Alternative names: | 1110001C14Rik Adipose triglyceride lipase ATGL ATGL DESNUTRIN Calcium independent phospholipase A2 Calcium-independent phospholipase A2 Desnutrin EC 3.1.1.3 FP17548 IPLA2 zeta IPLA2-zeta Mutant patatin like phospholipase domain containing 2 Patatin like phospholipase domain containing 2 PATATIN LIKE PHOSPHOLIPASE DOMAIN CONTAINING PROTEIN 2 Patatin-like phospholipase domain-containing protein 2 PEDF R PHOSPHOLIPASE A2 CALCIUM INDEPENDENT ZETA Pigment epithelium derived factor Pigment epithelium-derived factor plpl plpl2 PLPL2_HUMAN Pnpla2 Transport secretion protein 2 Transport secretion protein 2.2 Transport-secretion protein 2 Triglyceride hydrolase TTS 2.2 TTS2 TTS2.2 ZETA |
Images
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Fig1:
Western blot analysis of Adipose Triglyceride Lipase on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500301, 1/1,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: 293 cell lysate Lane 2: HepG2 cell lysate Lane 3: A431 cell lysate |
|
Fig2: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Adipose Triglyceride Lipase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500301, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.