Phospholipase C beta 1 Rabbit Polyclonal Antibody
cat.: HA500302
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 139 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Phospholipase C beta 1 aa 50-250 / 1216. |
| Positive control: | Rat brain tissue lysate, human brain tissue lysate, mouse brain tissue lysates, rat brain tissue, mouse brain tissue, SH-SY5Y. |
| Subcellular location: | Nucleus membrane, Cytoplasm. |
| Recommended Dilutions:
WB IHC-P FC |
1:1,000-1:5,000 1:100-1:200 1:100-1:500 |
| Uniprot #: | SwissProt: Q9NQ66 Human | Q9Z1B3 Mouse | P10687 Rat |
| Alternative names: | 1 phosphatidylinositol 4,5 bisphosphate phosphodiesterase beta 1 1-phosphatidyl-D-myo-inositol-4,5-bisphosphate 1-phosphatidylinositol 4 1-phosphatidylinositol-4 5-bisphosphate phosphodiesterase beta-1 EIEE12 Inositoltrisphosphohydrolase Monophosphatidylinositol phosphodiesterase Phosphb Phosphoinositidase C Phosphoinositide phospholipase C Phosphoinositide phospholipase C-beta 1 Phosphoinositide phospholipase C-beta-1 Phospholipase C beta 1 (phosphoinositide-specific) Phospholipase C I Phospholipase C-beta-1 Phospholipase C-I PI PLC PLC 1 PLC beta 1 PLC I PLC-154 PLC-beta-1 PLC-I PLC154 Plcb Plcb1 Plcb1 protein PLCB1_HUMAN PLCbeta1 Triphosphoinositide phosphodiesterase |
Images
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Fig1:
Western blot analysis of Phospholipase C beta 1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500302, 1/1,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Rat brain tissue lysate Lane 2: Human brain tissue lysate |
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Fig2: Western blot analysis of Phospholipase C beta 1 on mouse brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500302, 1/5,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
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Fig3: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Phospholipase C beta 1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500302, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Phospholipase C beta 1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500302, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5: Flow cytometric analysis of Phospholipase C beta 1 was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (HA500302, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.