Phospholipase C beta 1 Rabbit Polyclonal Antibody
cat.: HA500302
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, IF-Cell, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 139 kDa
Isotype: IgG
Immunogen: Recombinant protein within human Phospholipase C beta 1 aa 50-250 / 1216.
Positive control: Rat brain tissue lysate, Human brain tissue lysate, mouse brain tissue lysates, human brain tissue, mouse brain tissue, rat brain tissue, NIH/3T3, C6.
Subcellular location: Nucleus membrane, Cytoplasm.
Recommended Dilutions:
  WB
  IHC-P
  IF-Cell
  FC
1:1,000-1:5,000
1:100-1:200
1:100
1:1,000
Uniprot #: SwissProt: Q9NQ66 Human | Q9Z1B3 Mouse | P10687 Rat
Alternative names: 1 phosphatidylinositol 4,5 bisphosphate phosphodiesterase beta 1 1-phosphatidyl-D-myo-inositol-4,5-bisphosphate 1-phosphatidylinositol 4 1-phosphatidylinositol-4 5-bisphosphate phosphodiesterase beta-1 EIEE12 Inositoltrisphosphohydrolase Monophosphatidylinositol phosphodiesterase Phosphb Phosphoinositidase C Phosphoinositide phospholipase C Phosphoinositide phospholipase C-beta 1 Phosphoinositide phospholipase C-beta-1 Phospholipase C beta 1 (phosphoinositide-specific) Phospholipase C I Phospholipase C-beta-1 Phospholipase C-I PI PLC PLC 1 PLC beta 1 PLC I PLC-154 PLC-beta-1 PLC-I PLC154 Plcb Plcb1 Plcb1 protein PLCB1_HUMAN PLCbeta1 Triphosphoinositide phosphodiesterase
Images
HA500302_1.jpg Fig1: Western blot analysis of Phospholipase C beta 1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500302, 1/1,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Rat brain tissue lysate
Lane 2: Human brain tissue lysate
HA500302_2.jpg Fig2: Western blot analysis of Phospholipase C beta 1 on mouse brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500302, 1/5,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
HA500302_3.jpg Fig3: Immunocytochemistry analysis of NIH/3T3 cells labeling Phospholipase C beta 1 with Rabbit anti-Phospholipase C beta 1 antibody (HA500302) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospholipase C beta 1 antibody (HA500302) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
HA500302_4.jpg Fig4: Immunocytochemistry analysis of C6 cells labeling Phospholipase C beta 1 with Rabbit anti-Phospholipase C beta 1 antibody (HA500302) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospholipase C beta 1 antibody (HA500302) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
HA500302_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Phospholipase C beta 1 antibody (HA500302) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500302) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500302_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Phospholipase C beta 1 antibody (HA500302) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500302) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500302_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Phospholipase C beta 1 antibody (HA500302) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500302) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500302_8.jpg Fig8: Flow cytometric analysis of NIH/3T3 cells labeling Phospholipase C beta 1.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA500302, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
HA500302_9.jpg Fig9: Flow cytometric analysis of C6 cells labeling Phospholipase C beta 1.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA500302, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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