CX3CL1 Rabbit Polyclonal Antibody
cat.: HA500304
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 42 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human CX3CL1 aa 25-341 / 397 (Extracellular). |
| Positive control: | SK-BR-3 cell lysate, HepG2 cell lysate, human skin tissue, human breast carcinoma tissue. |
| Subcellular location: | Cell membrane, Membrane, Secreted. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:200 |
| Uniprot #: | SwissProt: P78423 Human |
| Alternative names: | A 152E5.2 AB030188 ABCD 3 ABCD3 AI848747 C-X3-C motif chemokine 1 C3Xkine Chemokine (C-X3-C motif) ligand 1 Chemokine C X3 C motif ligand 1 Chemokine CX3C Motif Ligand 1 CX3C membrane anchored chemokine CX3C membrane-anchored chemokine Cx3cl1 CXC 3 CXC3 CXC3C D8Bwg0439e FKN Fractalkine Neurotactin NTN NTT Processed fractalkine SCYD 1 SCYD1 Small inducible cytokine D1 Small inducible cytokine subfamily D (Cys X3 Cys) member 1 small inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine, neurotactin) Small inducible cytokine subfamily D member 1 Small-inducible cytokine D1 X3CL1_HUMAN |
Images
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Fig1:
Western blot analysis of CX3CL1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500304, 1/1,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SK-BR-3 cell lysate Lane 2: HepG2 cell lysate |
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Fig2: Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-CX3CL1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500304, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-CX3CL1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500304, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.