MMP-7 Rabbit Polyclonal Antibody
cat.: HA500305
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 42 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human MMP7 aa 220-267 / 267. |
| Positive control: | A549 cell lysates, HT-29 cell lysate, A549, human uterus tissue, HT-29. |
| Subcellular location: | Extracellular matrix, Secreted. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P FC |
1:500-1:2,000 1:50-1:200 1:50-1:200 1:50-1:100 1:500-1:1.000 |
| Uniprot #: | SwissProt: P09237 Human |
| Alternative names: | Matrilysin Matrin Matrix Metalloproteinase 7 Matrix metalloproteinase-7 MMP 7 MMP-7 MMP7 MMP7_HUMAN MPSL1 PUMP 1 Pump 1 protease Pump-1 protease PUMP1 Uterine matrilysin Uterine metalloproteinase |
Images
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Fig1:
Western blot analysis of MMP-7 on different lysates with Rabbit anti-MMP-7 antibody (HA500305) at 1/1,000 dilution. Lane 1: A549 (Human lung adenocarcinoma cell) cell lysate Lane 2: HT-29 (Human colorectal adenocarcinoma cell) cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 9 seconds ; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500305, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 29 kDa Observed band size: 27 kDa |
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Fig2: ICC staining of MMP-7 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500305, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3: Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-MMP7 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500305, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Flow cytometric analysis of MMP-7 was done on A549 cells. The cells were fixed, permeabilized and stained with the primary antibody (HA500305, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig5: ICC staining of MMP-7 in HT-29 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500305, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.